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Center for Molecular Medicine

Dedicated to understanding the intricacies of cellular signaling, the Center for Molecular Medicine was built on Caltech's successes at the interface of chemistry and biology. It is focused on determining how complex systems of molecules interact to create the pathways that regulate the lives of cells and allow them to respond to their environments. Rather than just studying a complex biomolecule in isolation, an understanding of how it is part of a larger path of molecules is the purpose of its establishment. The Center was made possible by a generous grant from the Gordon and Betty Moore Foundation.

If use of the Center instrumentation has contributed significantly to your research, we request that you cite the Center in the acknowledgments section of your publication. For example: "This work was supported by…, and by the Center for Molecular Medicine at California Institute of Technology."

Access to CM2 Instruments

  1. Please contact the appropriate caretakers for each instrument you would like to use for the instrument training.
  2. Upon completion of training, contact Dina Malounda ([email protected]) to get access.

People in the Facility

Faculty members: Dr. Dennis Dougherty, Dr. Jacqueline Barton, Dr. Peter Dervan, Dr. Linda Hsieh-Wilson, Dr. Shu-ou Shan, Dr. Alison Ondrus, Dr. Mikhail Shapiro

Safety Officer: Dina Malounda, [email protected]

Lab Assistants: Please see the table below for instrument caretakers

Seminars

Thursday afternoons
3:00 to 4:00 p.m.
Online only

Instrumentation

Crellin Room 262 (Bacterial)

flexstation

Microplate reader with 5 read modes (including absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence) with additional robot functionality (8-channel pipettor) to add liquid reagents.

Contact: John O. Chapman, ,[email protected]

Odyssey Li-Cor IR Imager

Quantitative scanning of western blots at 700 and 800 nm.

Contact: Ruilin Qian, [email protected]

Beckman LS 6000SC Scintillation Counter

The scintillation counter is designed to provide highly accurate, automated counting of the level of radioactivity in radioactivity-tagged samples. Instrument used to count radioactive samples. Note: New users must complete Radioactivity Safety Training with Haick Issaian ([email protected]), the Institute Radiation Safety Officer, before getting access to the scintillation counte.

Contact: Kausar Raza [email protected]

Nanodrop 2000

Micro-volume full spectrum UV-Visible spectrophotometer for sample volumes as low as 0.5 µL.

Contact: Li-En Lin, [email protected]

QIAcube

Fully automated sample preparation of Qiagen spin-column kits (up to 12 samples per run) for purification of DNA, RNA or proteins.

Contact: Xun Wang, [email protected]

I2500 KC Incubator Shaker

Floor-mounted shaker accepts up to 6-L flasks for culture.

Contact: Dina Malounda, [email protected]

Innova 43 Incubator Shaker

Floor-mounted temperature-controlled shaker accepts up to 6-L flasks for culture. Can go down to 5°C.

Contact: Dina Malounda, [email protected]

Avanti J-26 Centrifuge

Centrifuge up to 6 1-Liter cultures.

Contact: Alex Siegel, [email protected]

Ultrospec 10

Portable spectrophotometer for OD600 readings.

Contact: Terry Kim, [email protected]

-80°C freezer

Intended for use as a back-up of individual lab freezers.

Contact: Dina Malounda [email protected]

Crellin Room 260 (Mammalian)

Typhoon FLA 9000

High-resolution biomolecular imager capable of detecting storage phosphor, chemiluminescence, and multiplex fluorescence with 473 nm, 532 nm, and 625 nm excitation. Analysis done with ImageQuant software.

Contact: Helena Awad, [email protected]

Amaxa 4D-Nucleofector

Efficient electroporation of mammalian cells for transfection.

Contact: James Linton, [email protected]

CFX 96 Real-Time PCR Detection System

Built upon the Bio-Rad C1000 96 well Thermal Cycler, utilizes filtered LEDs for excitation and is capable of monitoring 450-750 nM excitation/emission wavelength range. This system allows for simultaneous detection of up to 5 different fluorophores per reaction and is sensitive enough to detect one copy of target sequence in human genomic DNA.

Contact: Boyoung Shin [email protected] and Tom Sidwell [email protected]

Neon Transfection System

High efficiency electroporation transfection device for primary cells, stem cells, and difficult-to-transfect cells. It is capable of transfecting 10 or 100 µL volumes.

Contact: Raymond J. Zhang, [email protected]

Nikon SMZ1000 Microscope

Used for dissections and imaging (transmitted light) with the camera attached. Zoom from 0.8 to 8X, in a clean bench to provide a sterile dissection/preparation work space.

Contact: Terry Kim,[email protected]

Nikon TS100 Microscope

Used for basic visualization of cultured cells at 10 and 20X, allowing for monitoring and counting cells.

Contact: Kun Mmiao, [email protected]

Clean Bench

Provides sterile environment for sensitive dissections or microscope work.

Contact: Terry Kim, [email protected]

Laminar Flow Hoods/Biosafety Cabinets

Provides sterile environment for mammalian cell work.

Contact: Alice Hsu, [email protected]

Mammalian Tissue Culture CO2 Incubator

Provides a controlled CO2 and temperature environment for cells during dissection, transfection or passaging done at the Center. It is intended for temporary use, and not long term housing of cultured cells.

Contact: Kun Miao, [email protected]

Tabletop centrifuge that can accomodate 15 or 50 mL conical tubes.

Contact: Dina Malounda, [email protected]

Contact: Alice Hsu, [email protected]

Instrumentation Caretakers

For training or troubleshooting, please contact the appropriate caretakers.

Equipment Caretaker Contact info
Biosafety cabinet (TC hood) Alice Hsu [email protected]
Chemical fume hood Dina Malounda [email protected]
Amaxa 4-D Nucleofector James Lilton [email protected]
Benchtop centrifuges Dina Malounda [email protected]
Benchtop water bath Alice Hsu [email protected]
MilliQ water deionizer Amylynn Chen [email protected]
Typhoon FLA 9000 gel imager Helena Awad [email protected]
Inverted fluorescence microscope (Nikon TS100) Kun Miao [email protected]
Upright dissection microscope (Nikon SMZ1000) + Clean bench Terry Kim [email protected]
Affinity ITC Chia-Yu Chien [email protected]
Mammalian cell incubator Kun Miao [email protected]
Zeta Potential Analyzer Bill Ling [email protected]
BioRad qPCR (CFX 96 RT PCR) Boyoung Shin and Tom Sidwell [email protected], [email protected]
4°C refrigerator Alice Hsu [email protected]
FlexStation microplate reader John O. Chapman [email protected]
Beckman Avanti J-26 floor centrifuge Alex Siegel [email protected]
-80°C freezer Dina Malounda [email protected]
Nanodrop 2000 Li-En Lin [email protected]
Li-Cor IR imager Ruilin Qian [email protected]
Beckman Scintillation Counter Kausar Raza [email protected]
Shaking incubator for bacteria Dina Malounda [email protected]
Ultrospec cell density meter Terry Kim [email protected]
QIAcube Xun Wang [email protected]
Neon transfection system Raymond J. Zhang [email protected]
Ice maker Trixia Buscagan [email protected]

Mailing address

Caltech Center for Molecular Medicine
Division of Chemistry and Chemical Engineering
MC 164-30
California Institute of Technology
Pasadena, CA 91125
USA