Ch 7

Advanced Experimental Methods in Bioorganic Chemistry

9 units (1-6-2)  |  third term
Prerequisites: Ch 41 abc, and Ch/Bi 110, Ch 4 ab. Enrollment by instructor's permission.
Preference will be given to students who have taken Ch 5 a or Bi 10. This advanced laboratory course will provide experience in powerful contemporary methods used in chemical biology, including polypeptide synthesis and the selective labeling and imaging of glycoproteins in cells. Experiments will address amino acid protecting group strategies, biopolymer assembly and isolation, and product characterization. A strong emphasis will be placed on understanding the chemical basis underlying the successful utilization of these procedures. In addition, experiments to demonstrate the application of commercially available enzymes for useful synthetic organic transformations will be illustrated.
Instructor: Hsieh-Wilson

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